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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1200| Visualizing individual microtubules by bright field microscopy | |
| BRAULIO GUTIERREZ MEDINA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1119/1.3453264 | |
| "Microtubules are slender (similar to 25 nm diameter), filamentous polymers involved in cellular structure and organization. Individual microtubules have been visualized via fluorescence imaging of dye-labeled tubulin subunits and by video-enhanced, differential interference-contrast microscopy of unlabeled polymers using sensitive CCD cameras. We demonstrate the imaging of unstained microtubules using a microscope with conventional bright field optics in conjunction with a webcam-type camera and a light-emitting diode illuminator. The light scattered by microtubules is image-processed to remove the background, reduce noise, and enhance contrast. The setup is based on a commercial microscope with a minimal set of inexpensive components, suitable for implementation in a student laboratory. We show how this approach can be used in a demonstration motility assay, tracking the gliding motions of microtubules driven by the motor protein kinesin." | |
| American Association of Physics Teachers, American Institute of Physics | |
| 2010-11 | |
| Artículo | |
| Inglés | |
| Público en general | |
| Gutierrez-Medina, B, & Block, Steven M. American Journal of Physics 78, 1152 (2010); https://doi.org/10.1119/1.3453264 | |
| FÍSICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Nanociencias y Materiales |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| AmJPhys78(2010)1152.pdf | 1.03 MB | Adobe PDF | Visualizar/Abrir |