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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1303| Evaluation of a SUMO E2 conjugating enzyme involved in resistance to Clavibacter michiganensis subsp. michiganensis in Solanum peruvianum, through a tomato mottle virus VIGS assay | |
| MAYRA JANETH ESPARZA ARAIZA BERNARDO BAÑUELOS HERNANDEZ GERARDO RAFAEL ARGUELLO ASTORGA JOSE PABLO LARA AVILA DULCE ALEJANDRA ROUGON CARDOSO ANGEL GABRIEL ALPUCHE SOLIS | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.3389/fpls.2015.01019 | |
| SUMO-conjugating enzyme Virus-induced gene-silencing Tomato Mottle Virus Bacterial canker Clavibacter michiganensis subsp. michiganensis | |
| "Clavibacter michiganensis subsp. michiganensis (Cmm) causes bacterial wilt and canker of tomato. Currently, no Solanum lycopersicum resistant varieties are commercially available, but some degree of Cmm resistance has been identified in Solanum peruvianurn. Previous research showed up regulation of a SUMO E2 conjugating enzyme (SCEI) transcript in S. peruvianum compared to S. lycopersicum following infection with Cmm. In order to test the role of SCEI in resistance to Cmm, a fragment of SCEI from S. peruvianum was cloned into a novel virus induced gene silencing (VIGS) vector based on the geminivirus, Tomato Mottle Virus (ToMoV). Using biolistic inoculation, the ToMoV-based VIGS vector was shown to be effective in S. peruvianum by silencing the magnesium chelatase gene, resulting in leaf bleaching. VIGS with the ToMoV_SCEI construct resulted in 61% silencing of SCEI in leaves of S. peruvianum as determined by quantitative RT-PCR. The SCEI -silenced plants showed unilateral wilting (15 dpi) and subsequent death (20 dpi) of the entire plant after Cmm inoculation, whereas the empty vector-treated plants only showed wilting in the Cmm-inoculated leaf. The SCEI-silenced plants showed higher Cmm colonization and an average of 4.5 times more damaged tissue compared to the empty vector control plants. SCEI appears to play an important role in the innate immunity of S. peruvianum against Cmm, perhaps through the regulation of transcription factors, leading to expression of proteins involved in salicylic acid dependent defense responses." | |
| Frontiers Research Foundation | |
| 2015-12 | |
| Artículo | |
| Inglés | |
| Público en general | |
| Esparza-Araiza MJ, Bañuelos-Hernández B, Argüello-Astorga GR, Lara-Ávila JP, Goodwin PH, Isordia-Jasso MI, Castillo-Collazo R, Rougon-Cardoso A and Alpuche-Solís ÁG. (2015) Evaluation of a SUMO E2 Conjugating Enzyme Involved in Resistance to Clavibacter michiganensis Subsp. michiganensis in Solanum peruvianum, Through a Tomato Mottle Virus VIGS Assay. Front. Plant Sci. 6:1019. doi: 10.3389/fpls.2015.01019 | |
| CIENCIAS DE LA VIDA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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