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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1305| Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening | |
| MITZUKO DAUTT CASTRO ADRIAN OCHOA LEYVA CARMEN ARMINDA CONTRERAS VERGARA MAGDA ADELINA PACHECO SANCHEZ J SERGIO CASAS FLORES FIDEL ALEJANDRO SANCHEZ FLORES MARIA AUXILIADORA ISLAS OSUNA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| http://dx.doi.org/10.3389/fpls.2015.00062 | |
| Mangifera indica L. Mesocarp Fruit ripening Transcriptome Cell wall hydrolytic enzymes Ethylene Fruit quality | |
| "Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR <= 0.05)1. The expression of 10 genes evaluated by both gRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening." | |
| Frontiers Research Foundation | |
| 2015-02 | |
| Artículo | |
| Inglés | |
| Público en general | |
| Dautt-Castro M, Ochoa-Leyva A, Contreras-Vergara CA, Pacheco-Sanchez MA, Casas-Flores S, Sanchez-Flores A, Kuhn DN and Islas-Osuna MA (2015) Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening. Front. Plant Sci. 6:62. doi: 10.3389/fpls.2015.00062 | |
| CIENCIAS DE LA VIDA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| FrontiersPlantScience6(2015)62.pdf | 2.64 MB | Adobe PDF | Visualizar/Abrir |