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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1491| Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites | |
| Antonio de León Rodríguez Santiago L Caño Muñiza Junyang Liu David K. Summers | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.nbt.2016.09.005 | |
| Bacteriostatic Escherichia coli Hydrogen Ethanol Indole Ionophore Lactate | |
| "Indole is a bicyclic signaling molecule with effects on both eukaryotic and prokaryotic cells. The majority of studies of indole action have been performed with bacteria cultured under aerobic conditions and little information is available about its effects under anaerobic conditions. Here the effect of the indole on anaerobic metabolism of Escherichia coli WDHL was studied. Indole in the range 0.5–8 mM was added to the culture medium and cell growth, hydrogen and metabolite production were compared to cultures lacking indole. Results showed that while 8 mM indole abolished growth completely, 4 mM indole had a partial bacteriostatic effect and the maximum optical density of the culture decreased by 44% compared to the control cultures. In addition, 4 mM indole had an important effect on anaerobic metabolism. Hydrogen production increased from 650 ± 115 to 1137 ± 343 mL H2/L, and hydrogen yield increased from 0.45 ± 0.1 to 0.94 ± 0.34 mol H2/mol glucose, compared to the control culture. Carbon flux was also affected and the composition of the final by-products changed. Lactate (41 mM) was the main metabolite in the control cultures, whereas ethanol (56.2 mM) and acetate (41.2 mM) were the main metabolites in the cultures with 2 mM indole. We conclude that the supplementation of E. coli cultures with exogenous indole is a simple and novel strategy to improve the production of hydrogen as well as other metabolites such as ethanol used as biofuels." | |
| Elsevier Science BV | |
| 2016-12 | |
| Artículo | |
| Antonio De Leon-Rodriguez, Santiago L. Caño-Muñiz, Junyan Liu, David K. Summers, Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites, New Biotechnology, Volume 33, Issue 6, 2016, Pages 868-873, ISSN 1871-6784. | |
| BIOLOGÍA MOLECULAR | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| NewBiotechnol33(2016)868.pdf | 223.98 kB | Adobe PDF | Visualizar/Abrir |