Por favor, use este identificador para citar o enlazar este ítem: http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1844
Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins
MARIA GLORIA SOLIS GUZMAN
Gerardo Rafael Argüello Astorga
JOSE LOPEZ BUCIO
LEON FRANCISCO RUIZ HERRERA
Joel Edmundo López Meza
LENIN SANCHEZ CALDERON
YAZMIN CARREON ABUD
MIGUEL MARTINEZ TRUJILLO
Acceso Abierto
Atribución-NoComercial-SinDerivadas
http://dx.doi.org/10.1590/1678-4685-gmb-2016-0223
Spen
Arabidopsis
Vascular bundle
Expression
"Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its promoter region with uidA::gfp reporter genes; the expression was observed in vascular tissues of leaves and roots, as well as in ovules and developing embryos. There was absence of a notable phenotype in knockout and overexpressing lines, suggesting that its function in plants might be specific to certain endogenous or environmental conditions. Our results suggest that the function of Atspen2 diverged from that of fpa due in part to their different transcription expression pattern and divergence of the regulatory SPOC domain."
Sociedade Brasileira de Genética
2017
Artículo
Solís-Guzmán, María Gloria, Argüello-Astorga, Gerardo, López-Bucio, José, Ruiz-Herrera, León Francisco, López-Meza, Joel, Sánchez-Calderón, Lenin, Carreón-Abud, Yazmín, & Martínez-Trujillo, Miguel. (2017). Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins. Genetics and Molecular Biology, 40(3), 643-655. Epub August 28, 2017
BIOLOGÍA MOLECULAR
Versión publicada
publishedVersion - Versión publicada
Aparece en las colecciones: Publicaciones Científicas Biología Molecular

Cargar archivos:


Fichero Tamaño Formato  
GenetMolBiol40(2017)643.pdf3.63 MBAdobe PDFVisualizar/Abrir