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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1976| Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress | |
| Rosalia Cristina Paz Rubén Anibal Rocco JUAN FRANCISCO JIMENEZ BREMONT Margarita Rodríguez y Domínguez Kessler ALICIA BECERRA FLORA Ana Bernardina Menéndez Oscar Adolfo Ruíz | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.plaphy.2014.06.009 | |
| Alkaline stress Adaptive response Lotus tenuis Long-term response Root and shoot tissues Suppressive Subtractive Hybridization Stress induced transcripts | |
| "Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L. tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10 mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L. tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants." | |
| Elsevier | |
| 2014 | |
| Artículo | |
| Rosalía Cristina Paz, Rubén Anibal Rocco, Juan Francisco Jiménez-Bremont, Margarita Rodríguez-Kessler, Alicia Becerra-Flora, Ana Bernardina Menéndez, Oscar Adolfo Ruíz, Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress, Plant Physiology and Biochemistry, Volume 82, 2014, Pages 279-288. | |
| BIOLOGÍA MOLECULAR | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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