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Optimization of biohydrogen production by the novel psychrophilic strain N92 collected from the Antarctica
SERGIO CISNEROS DE LA CUEVA
CECILIA LIZETH ALVAREZ GUZMAN
VICTOR EMMANUEL BALDERAS HERNANDEZ
Antonio de León Rodríguez
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.1016/j.ijhydene.2017.11.164
Biohydrogen
Central composite design
Dark fermentation
Psychrophilic bacteria
Response surface methodology
"In this study, the response surface methodology (RSM) with central composite design (CCD) was employed to improve the hydrogen production by the psychrophilic N92 strain (EU636058) isolated from Antarctica, which is closely related to Pseudorhodobacter sp. (KT163920). The influence of operational conditions such as temperature (4.7–55.2 °C), initial pH (3.44–10.16), and initial glucose concentration (4.7–55.23 g/dm3), as well as the initial concentrations of (NH4)2SO4 (0.05–3.98 g/dm3), FeSO4 (0.02–1.33 g/dm3) and NaHCO3 (0.02–3.95 g/dm3) was evaluated. The linear effect of glucose concentration, along with the quadratic effect of all the six factors were the most significant terms affecting the biohydrogen yield by N92 strain. The optimum conditions for the maximum hydrogen yield of 1.7 mol H2/mol glucose were initial pH of 6.86, glucose concentration of 28.4 g/dm3, temperature 29 °C and initial concentration of (NH4)2SO4, FeSO4 and NaHCO3 of 0.53, 1.55 and 1.64 g/dm3 respectively. Analysis of the metabolites produced under the optimum conditions showed that the most abundant were acetic acid (0.8 g/dm3), butyric acid (0.7 g/dm3) and ethanol (2.1 g/dm3). We suggest that the bioprocess established in this study using the strain N92 could be an alternative for hydrogen production with the advantages of constituting low energy costs in fermentation."
Elsevier
2018
Artículo
Sergio Cisneros de la Cueva, Cecilia L. Alvarez Guzmán, Víctor E. Balderas Hernández, Antonio De León Rodríguez, Optimization of biohydrogen production by the novel psychrophilic strain N92 collected from the Antarctica, International Journal of Hydrogen Energy, Volume 43, Issue 30, 2018, Pages 13798-13809, https://doi.org/10.1016/j.ijhydene.2017.11.164
BIOLOGÍA Y QUÍMICA
Versión revisada
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Aparece en las colecciones: Publicaciones Científicas Biología Molecular

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