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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2152| Secretion of an argonaute protein by a parasitic nematode and the evolution of its siRNA guides | |
| Wang-ngai Chow Georgios Koutsovoulos CESARE MOISES OVANDO VAZQUEZ Kyriaki Neophytou JOSE ROBERTO BERMUDEZ BARRIENTOS Dominik Laetsch Elaine Robertson Sujai Kumar Julie M Claycomb Mark Blaxter Cei Abreu_Goodger Amy Buck | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1093/nar/gkz142 | |
| Web server RNA Annotation | |
| "Extracellular RNA has been proposed to mediate communication between cells and organisms however relatively little is understood regarding how specific sequences are selected for export. Here, we describe a specific Argonaute protein (exWAGO) that is secreted in extracellular vesicles (EVs) released by the gastrointestinal nematode Heligmosomoides bakeri, at multiple copies per EV. Phylogenetic and gene expression analyses demonstrate exWAGO orthologues are highly conserved and abundantly expressed in related parasites but highly diverged in free-living genus Caenorhabditis. We show that the most abundant small RNAs released from the nematode parasite are not microRNAs as previously thought, but rather secondary small interfering RNAs (siRNAs) that are produced by RNA-dependent RNA Polymerases. The siRNAs that are released in EVs have distinct evolutionary properties compared to those resident in free-living or parasitic nematodes. Immunoprecipitation of exWAGO demonstrates that it specifically associates with siRNAs from transposons and newly evolved repetitive elements that are packaged in EVs and released into the host environment. Together this work demonstrates molecular and evolutionary selectivity in the small RNA sequences that are released in EVs into the host environment and identifies a novel Argonaute protein as the mediator of this." | |
| Oxford University Press | |
| 2019 | |
| Artículo | |
| Franklin Wang-Ngai Chow, Georgios Koutsovoulos, Cesaré Ovando Vázquez, Kyriaki Neophytou, Jose R Bermúdez-Barrientos, Dominik R Laetsch, Elaine Robertson, Sujai Kumar, Julie M Claycomb, Mark Blaxter, Cei Abreu-Goodger, Amy H Buck, Secretion of an Argonaute protein by a parasitic nematode and the evolution of its siRNA guides, Nucleic Acids Research, Volume 47, Issue 7, 23 April 2019, Pages 3594-3606, https://doi.org/10.1093/nar/gkz142 | |
| BIOQUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Nanociencias y Materiales |
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| NucleicAcidsResearch47(2019)3594.pdf | 3.12 MB | Adobe PDF | Visualizar/Abrir |