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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1165| Molecular and biochemical characterization of extracellular tannin acyl hydrolase activity from a Mexican isolate of Aspergillus niger | |
| MA. FABIOLA LEON GALVAN IRINEO TORRES PACHECO LORENZO GUEVARA OLVERA ANA PAULINA BARBA DE LA ROSA MARIO MARTIN GONZALEZ CHAVIRA RAMON GERARDO GUEVARA GONZALEZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Tannase Aspergillus niger Guanajuato isolate cDNA cloning | |
| "Microbial tannase, a hydrolysable tannin-degrading enzyme, is extensively used in manufacture of instant tea, beer, wine, and gallic acid. Aspergillus niger strain, obtained from a Mexican tannery wastewaters rich in gallic acid [Quebracho Phenolics-rich Tannery Wastewaters, (QPTW)], displayed a good growth and tannase activity in a minimal medium added with 1% (w/v) QPTW (Kr= 0.451 mm.h-1). Using PCR and RACE 3´ and 5´methodologies, a complete cDNA of a tannase was cloned from this isolate.Nucleotide sequence of complete cDNA was of 4690 bp with a complete ORF of 1833 bp encoding 611 amino acids. Transcriptionalinduction was observed in mineral medium added with carbon sources as tannic acid alone (1 and 10 g/l), as well as mix of glucose(1 and 10 g/l) and tannic acid (1 g/l) in the media. However, neither glucose (1 and 10 g/l) and sucrose (1 and 10 g/l) nor (+)-catechin(1 and 10 g/l) as sole carbon sources displayed gene induction in in vitro assays. A. niger-GTO is a new strain with interesting characteristics for industrial tannase production purposes." | |
| National Institute of Science Communication And Information Resources | |
| 2010-12 | |
| Artículo | |
| Inglés | |
| Público en general | |
| INGENIERÍA Y TECNOLOGÍA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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