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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1271| A minimal optical trapping and imaging microscopy system | |
| CARMEN NOEMI HERNANDEZ CANDIA SARA TAFOYA MARTINEZ BRAULIO GUTIERREZ MEDINA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1371/journal.pone.0057383 | |
| "We report the construction and testing of a simple and versatile optical trapping apparatus, suitable for visualizing individual microtubules (similar to 25 nm in diameter) and performing single-molecule studies, using a minimal set of components. This design is based on a conventional, inverted microscope, operating under plain bright field illumination. A single laser beam enables standard optical trapping and the measurement of molecular displacements and forces, whereas digital image processing affords real-time sample visualization with reduced noise and enhanced contrast. We have tested our trapping and imaging instrument by measuring the persistence length of individual double-stranded DNA molecules, and by following the stepping of single kinesin motor proteins along clearly imaged microtubules. The approach presented here provides a straightforward alternative for studies of biomaterials and individual biomolecules." | |
| Public Library of Science | |
| 2013-02 | |
| Artículo | |
| Inglés | |
| Público en general | |
| Hernández Candia CN, Tafoya Martínez S, Gutiérrez-Medina B (2013) A Minimal Optical Trapping and Imaging Microscopy System. PLoS ONE 8(2): e57383. https://doi.org/10.1371/journal.pone.0057383 | |
| OTRAS ESPECIALIDADES TECNOLÓGICAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Nanociencias y Materiales |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| PLoSONE8(2013)e57383.PDF | 2.08 MB | Adobe PDF | Visualizar/Abrir |