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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1306| Nuclear localization of the dehydrin OpsDHN1 is determined by histidine-rich motif | |
| ITZELL EURIDICE HERNANDEZ SANCHEZ ISRAEL MARURI LOPEZ Juan Carbonell JUAN FRANCISCO JIMENEZ BREMONT | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.3389/fpls.2015.00702 | |
| Dehydrin BiFC Homodimer Histidine-rich motif Nuclear/cytoplasmic localization | |
| "The cactus OpsDHN1 dehydrin belongs to a large family of disordered and highly hydrophilic proteins known as Late Embryogenesis Abundant (LEA) proteins, which accumulate during the late stages of embryogenesis and in response to abiotic stresses. Herein, we present the in vivo OpsDHN1 subcellular localization by N-terminal GFP translational fusion; our results revealed a cytoplasmic and nuclear localization of the GFP::OpsDHN1 protein in Nicotiana benthamiana epidermal cells. In addition, dimer assembly of OpsDHN1 in planta using a Bimolecular Fluorescence Complementation (BiFC) approach was demonstrated. In order to understand the in vivo role of the histidine-rich motif, the OpsDHN1 - Delta His version was produced and assayed for its subcellular localization and dimer capability by GFP fusion and BiFC assays, respectively. We found that deletion of the OpsDHN1 histidine-rich motif restricted its localization to cytoplasm, but did not affect dimer formation. In addition, the deletion of the S-segment in the OpsDHN1 protein affected its nuclear localization. Our data suggest that the deletion of histidine-rich motif and S-segment show similar effects, preventing OpsDHN1 from getting into the nucleus. Based on these results, the histidine-rich motif is proposed as a targeting element for OpsDHN1 nuclear localization." | |
| Frontiers Research Foundation | |
| 2015-09 | |
| Artículo | |
| Inglés | |
| Público en general | |
| Hernández-Sánchez IE, Maruri-López I, Ferrando A, Carbonell J, Graether SP and Jiménez-Bremont JF (2015) Nuclear localization of the dehydrin OpsDHN1 is determined by histidine-rich motif. Front. Plant Sci. 6:702. doi: 10.3389/fpls.2015.00702 | |
| QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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