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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1467| Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta | |
| Luz María Teresita Paz Maldonado VICTOR EMMANUEL BALDERAS HERNANDEZ Emilio Medina Rivero Ana Paulina Barba de la Rosa JOSE LUIS FLORES FLORES LEANDRO GABRIEL ORDOÑEZ ACEVEDO Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.bioeng.2006.10.001 | |
| Codon bias E. coli BL21-SI Multiple sclerosis Response surface methodology (RSM) Synthetic gene Yeast extract | |
| "A human interferon beta (hINF-β) synthetic gene was optimized and expressed in Escherichia coli BL21-SI using a vector with the T7 promoter. To determine the best culture conditions such as culture medium, temperature, cell density and inducer concentration, we used the response surface methodology and a Box-Behnken design to get the highest hINF-β production. The maximum hINF-β production of 61 mg l−1 was attained using minimum medium and the following predicted optimal conditions: temperature of 32.5 °C, cell density of 0.64, and inducer concentration of 0.30 M NaCl. This is the first report showing the successful performance of the BL21-SI system in a minimum medium. The response surface methodology is effective for the optimization of recombinant protein production using synthetic genes." | |
| Elsevier Science BV | |
| 2007-06 | |
| Artículo | |
| Luz M.T. Paz Maldonado, Víctor E. Balderas Hernández, Emilio Medina Rivero, Ana P. Barba de la Rosa, José L. Flores Flores, Leandro G. Ordoñez Acevedo, Antonio De León Rodríguez, Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: The case of human interferon beta, Biomolecular Engineering, Volume 24, Issue 2, 2007, Pages 217-222. | |
| BIOLOGÍA MOLECULAR | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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