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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1468| Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli | |
| Emilio Medina Rivero VICTOR EMMANUEL BALDERAS HERNANDEZ LEANDRO GABRIEL ORDOÑEZ ACEVEDO Luz María Teresita Paz Maldonado Ana Paulina Barba de la Rosa Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1007/s10529-007-9395-5 | |
| Periplasm Signal peptide Tat pathway Therapeutic protein | |
| "Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction." | |
| Springer | |
| 2007-09 | |
| Artículo | |
| Medina-Rivero, E., Balderas-Hernández, V.E., Ordoñez-Acevedo, L.G. et al. Biotechnol Lett (2007) 29: 1369. https://doi.org/10.1007/s10529-007-9395-5 | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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