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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1471| Optimization of human interferon gamma production in Escherichia coli by response surface methodology | |
| VICTOR EMMANUEL BALDERAS HERNANDEZ Luz María Teresita Paz Maldonado Emilio Medina Rivero Ana Paulina Barba de la Rosa LEANDRO GABRIEL ORDOÑEZ ACEVEDO Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1007/s12257-007-0126-5 | |
| Escherichia coli BL21-SI Minimum medium Therapeutic protein synthetic gene | |
| "The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, bio-mass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6°C, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli." | |
| The Korean Society for Biotechnology and Bioengineering | |
| 2008-02 | |
| Artículo | |
| Balderas Hernández, V.E., Paz Maldonado, L.M.T., Medina Rivero, E. et al. Biotechnol Bioproc E (2008) 13: 7. https://doi.org/10.1007/s12257-007-0126-5 | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| BiotechnolBioprocE13(2008)7.pdf | 210.37 kB | Adobe PDF | Visualizar/Abrir |