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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1474| Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica | |
| SERGIO ANGEL GARCIA ECHAURI Manuel Gidekel Ana Gutiérrez Moraga LEANDRO GABRIEL ORDOÑEZ ACEVEDO JUAN ANTONIO ROJAS CONTRERAS Ana Paulina Barba de la Rosa Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.procbio.2009.04.021 | |
| Antioxidant Plant Extremophil Psychrophilic enzyme Photo-oxidation Oxidative stress | |
| "Superoxide dismutase (SOD) catalyzes the conversion of the superoxide radical (O2−) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 °C. However, zymogram test showed that the enzyme has more activity at 4 °C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods." | |
| Elsevier Science LTD | |
| 2009-09 | |
| Artículo | |
| Sergio A. García Echauri, Manuel Gidekel, Ana Gutiérrez Moraga, Leandro G. Ordóñez, Juan A. Rojas Contreras, Ana P. Barba de la Rosa, Antonio De León Rodríguez, Heterologous expression of a novel psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica, Process Biochemistry, Volume 44, Issue 9, 2009, Pages 969-974. | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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