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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1475| Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis | |
| JUAN ANTONIO ROJAS CONTRERAS MARIO PEDRAZA REYES LEANDRO GABRIEL ORDOÑEZ ACEVEDO NORMA URTIZ ESTRADA Ana Paulina Barba de la Rosa Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.plasmid.2010.07.003 | |
| Heterologous Integrative Probiotic Therapeutic Recombinant Replicative plasmid and secretion | |
| "Integrative and replicative plasmids for the expression driven by the P43 promoter and secretion of recombinant proteins in Bacillus subtilis were constructed. The plasmids named pInt and pRep respectively were tested for the production of recombinant human interferon gamma (rhIFN-γ). A synthetic hIFN-γ gene employing the optimized B. subtilis codon usage was fused with the Bacillus licheniformis α-amylase signal peptide (sp-amyL) encoding sequence. The integrative construct produced 2.5 ± 0.2 mg l−1 and the replicative system produced 20.3 ± 0.8 mg l−1 of total recombinant rhIFN-γ. The results showed that secretion of hIFN-γ was the bottleneck for the overexpression of mature rhIFN-γ by B. subtilis." | |
| Elsevier B.V | |
| 2010-11 | |
| Artículo | |
| Juan A. Rojas Contreras, Mario Pedraza-Reyes, Leandro G. Ordoñez, Norma Urtiz Estrada, Ana P. Barba de la Rosa, Antonio De León-Rodríguez, Replicative and integrative plasmids for production of human interferon gamma in Bacillus subtilis, Plasmid, Volume 64, Issue 3, 2010, Pages 170-176. | |
| MICROBIOLOGÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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