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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1485| Novel fusion protein derived from vasostatin 30 and vasoinhibin II-14.1 potently inhibits coronary endothelial cell proliferation | |
| GABRIELA VAZQUEZ RODRIGUEZ María del Carmen González Castillo Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1007/s12033-012-9642-4 | |
| Angiogenesis Endothelium Cell proliferation Fusion protein Recombinant protein | |
| "Angiogenesis has been considered an important target for cancer therapy. The inhibition of angiogenesis represents a promising strategy for anti-cancer treatment, tumor growth inhibition, and metastasis. Vasostatin 30 (Vs30), and the 14.1 kDa vasoinhibin (Vi-II-14.1) are two peptides with remarkable anti-tumor and anti-angiogenic effect. The aim of this study was to produce a novel fusion protein between Vs30 and Vi-II-14.1, denominated VS_VI, to obtain a new protein with higher biological activity. The protein fusion genes were cloned into a T7 promoter-based vector, expressed in Escherichia coli BL21-SI and purified by affinity column chromatography. In vitro assays showed that the recombinant fusion protein inhibited rat coronary endothelial cell proliferation at 65.5 % at 10 nM, whereas recombinant Vs30 and Vi-II-14.1 inhibited at 33 and 50.5 % respectively, at the same concentration. The results showed that VS_VI is significantly more active than the Vs30 and Vi-II-14.1 separately. In addition, a practical classification of the vasoinhibins based on the peptide origin and theoretical molecular weight is proposed. This is the first study to produce a new fusion protein derived from Vs30 and Vi-II-14.1, both of them proposed as promising therapeutic agents." | |
| Humana Press Inc | |
| 2013-07 | |
| Artículo | |
| Vazquez Rodriguez, G., Gonzalez, C. & De Leon Rodriguez, A. Mol Biotechnol (2013) 54: 920. https://doi.org/10.1007/s12033-012-9642-4 | |
| BIOLOGÍA MOLECULAR | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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