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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1487| Expansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere | |
| Hera Andrade Zaldívar Marco Antonio Kalixto Sánchez Ana Paulina Barba de la Rosa Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1089/scd.2010.0236 | |
| "In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO2-free atmosphere were assessed. At day 5 of culture, total colony forming unit expansions of 14.44 ± 3.74, 11.20 ± 6.37, and 17.25 ± 3.65-folds were attained, respectively. The expansion reached using L-15 medium in roller bottles was around 10 times higher than that achieved in the static control cultures. To our knowledge, this is the first report of cultures in CO2-free atmosphere to expand cord blood human hematopoietic stem cells and it opens a new branch of possibilities for culturing and clinical applications." | |
| Mary Ann Liebert, Inc | |
| 2011-04 | |
| Artículo | |
| Hera Andrade-Zaldívar, Marco A. Kalixto-Sánchez, Ana P. Barba de la Rosa, and Antonio De León-Rodríguez.Stem Cells and Development.Apr 2011.ahead of printhttp://doi.org/10.1089/scd.2010.0236 | |
| BIOLOGÍA CELULAR | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| StemCellsDevelopment20(2011)593.pdf | 170.56 kB | Adobe PDF | Visualizar/Abrir |