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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1605| Histone deacetylase HDA-2 regulates Trichoderma atroviride growth, conidiation, blue light perception, and oxidative stress responses | |
| MACARIO OSORIO CONCEPCION GEMA ROSA CRISTOBAL MONDRAGON BRAULIO GUTIERREZ MEDINA J SERGIO CASAS FLORES | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| http://doi.org/10.1128/AEM.02922-16 | |
| Trichoderma Blue light Oxidative stress BLR proteins Histone acetylation Histone deacetylases HDA-2 Hos2p | |
| "Fungal blue-light photoreceptors have been proposed as integrators of light and oxidative stress. However, additional elements participating in the integrative pathway remain to be identified. In Trichoderma atroviride, the blue-light regulator (BLR) proteins BLR-1 and -2 are known to regulate gene transcription, mycelial growth, and asexual development upon illumination, and recent global transcriptional analysis revealed that the histone deacetylase-encoding gene hda-2 is induced by light. Here, by assessing responses to stimuli in wild-type and Δhda-2 backgrounds, we evaluate the role of HDA-2 in the regulation of genes responsive to light and oxidative stress. Δhda-2 strains present reduced growth, misregulation of the con-1 gene, and absence of conidia in response to light and mechanical injury. We found that the expression of hda-2 is BLR-1 dependent and HDA-2 in turn is essential for the transcription of early and late light-responsive genes that include blr-1, indicating a regulatory feedback loop. When subjected to reactive oxygen species (ROS), Δhda-2 mutants display high sensitivity whereas Δblr strains exhibit the opposite phenotype. Consistently, in the presence of ROS, ROS-related genes show high transcription levels in wild-type and Δblr strains but misregulation in Δhda-2 mutants. Finally, chromatin immunoprecipitations of histone H3 acetylated at Lys9/Lys14 on cat-3 and gst-1 promoters display low accumulation of H3K9K14ac in Δblr and Δhda-2 strains, suggesting indirect regulation of ROS-related genes by HDA-2. Our results point to a mutual dependence between HDA-2 and BLR proteins and reveal the role of these proteins in an intricate gene regulation landscape in response to blue light and ROS." | |
| American Society for Microbiology | |
| 2017-02 | |
| Artículo | |
| Osorio-Concepción, M., Cristóbal-Mondragón, G. R., Gutiérrez-Medina, B., & Casas-Flores, S. (2017). Histone Deacetylase HDA-2 Regulates Trichoderma atroviride Growth, Conidiation, Blue Light Perception, and Oxidative Stress Responses. Applied and Environmental Microbiology, 83(3), e02922–16. http://doi.org/10.1128/AEM.02922-16 | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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