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Arabidopsis thaliana sucrose phosphate synthase (sps) genes are expressed differentially in organs and tissues, and their transcription is regulated by osmotic stress
MARIA GLORIA SOLIS GUZMAN
GERARDO RAFAEL ARGUELLO ASTORGA
JOSE LOPEZ BUCIO
LEON FRANCISCO RUIZ HERRERA
JOEL EDMUNDO LOPEZ MEZA
LENIN SANCHEZ CALDERON
YAZMIN CARREON ABUD
MIGUEL MARTINEZ TRUJILLO
Acceso Abierto
30-11-2018
Atribución-NoComercial-SinDerivadas
https://doi.org/10.1016/j.gep.2017.06.001
Sucrose
Genes
Transcription
Osmotic stress
"Sucrose is synthesized from UDP-Glc and Fru-6-phosphate via the activity of sucrose-phosphate synthase (SPS) enzymes, which produce Suc-6-phosphate. Suc-6-phosphate is rapidly dephosphorylated by phosphatases to produce Suc and inorganic phosphate. Arabidopsis has four sps genes encoding SPS enzymes. Of these enzymes, AtSPS1F and AtSPS2F have been grouped with other dicotyledonous SPS enzymes, while AtSPS3F and AtSPS4F are included in groups with both dicotyledonous and monocotyledonous SPS enzymes. In this work, we generated Arabidopsis thaliana transformants containing the promoter region of each sps gene fused to gfp::uidA reporter genes. A detailed characterization of expression conferred by the sps promoters in organs and tissues was performed. We observed expression of AtSPS1F, AtSPS2F and AtSPS3F in the columella roots of the plants that support sucrose synthesis. Hence, these findings support the idea that sucrose synthesis occurs in the columella cells, and suggests that sucrose has a role in this tissue. In addition, the expression of AtSPS4F was identified in embryos and suggests its participation in this developmental stage. Quantitative transcriptional analysis of A. thaliana plants grown in media with different osmotic potential showed that AtSPS2F and AtSPS4F respond to osmotic stress."
Elsevier B.V.
2017-11
Artículo
María Gloria Solís-Guzmán, Gerardo Argüello-Astorga, José López-Bucio, León Francisco Ruiz-Herrera, Joel Edmundo López-Meza, Lenin Sánchez-Calderón, Yazmín Carreón-Abud, Miguel Martínez-Trujillo, Arabidopsis thaliana sucrose phosphate synthase (sps) genes are expressed differentially in organs and tissues, and their transcription is regulated by osmotic stress, Gene Expression Patterns, Volumes 25–26, 2017, Pages 92-101.
BIOLOGÍA MOLECULAR
Versión aceptada
acceptedVersion - Versión aceptada
Aparece en las colecciones: Publicaciones Científicas Biología Molecular

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