Por favor, use este identificador para citar o enlazar este ítem:
http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1919| Self-association of enolase from Trichomonas vaginalis. monomers, dimers, and octamers coexist in solution | |
| ELIBETH MIRASOL MELENDEZ ENRIQUE JAIME LIMA MUÑOZ Víctor Hugo Lara Luis Gabriel Brieba de Castro SAMUEL LARA GONZALEZ CLAUDIA GUADALUPE BENITEZ CARDOZA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| http://dx.doi.org/10.1021/acsomega.8b02197 | |
| Plasminogen-binding Yeast enolase Triosephosphate isomerase Streptococcus-pneumoniae Quaternary structure Mammalian enolases Alpha enolase Protein Dissociation Scattering | |
| "We used small-angle X-ray scattering to study the self-association of enolase from Trichomonas vaginalis as a function of the protein concentration and cosolute type. We observed coexisting monomers, dimers, and octamers in variable relative populations, depending on whether Tris–acetate, Tris–HCl, or potassium phosphate buffers were used. Phosphate ions hindered the formation of dimers and octamers. In contrast, the populations of dimers and octamers increased in Tris–acetate or Tris–HCl buffers and additionally increased by augmenting protein concentration or adding magnesium. Single oligomeric species could not be isolated in any of the experimental conditions tested. Furthermore, the secondary and tertiary structures, as well as the temperature-induced denaturation of the mixtures of species, were investigated. The acquired species lost enzymatic activity, but they were prone to interact with plasminogen, as judged from changes in the secondary and tertiary structures upon complex formation." | |
| American Chemical Society | |
| 2018 | |
| Artículo | |
| ACS Omega 2018, 3, 12, 17871-17880 | |
| QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| ACSOmega3(2018)17871.pdf | 3.94 MB | Adobe PDF | Visualizar/Abrir |