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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1945| Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets | |
| JUAN DIEGO CORTES GARCIA CINTYA DEL REFUGIO LOPEZ LOPEZ NANCY CORTEZ ESPINOSA MARIANA HAYDEE GARCIA HERNANDEZ JUAN MANUEL GUZMAN FLORES ESTHER LAYSECA ESPINOSA LILIANA PORTALES CERVANTES DIANA PATRICIA PORTALES PEREZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.imbio.2015.07.018 | |
| P2X7 receptor ART1 ATP NAD Immune regulation CD39 | |
| "Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can activate the P2X7 receptor via ADP-ribosyltransferase (ART) enzymes and cause cell depletion in murine models. We evaluated the expression and function of P2X7 and ART1 in CD39+ Treg and CD39- Treg cells in the presence or absence of ATP and NAD. We isolated peripheral blood mononuclear cells from healthy subjects and purified CD4+ T cells, CD4+ CD25+ T cells and CD4+ CD25+ CD39+ T cells. P2X7 and ART1 expression was assessed by flow cytometry and real-time PCR. Our results showed low P2X7 expression on CD39+ Treg cells and higher levels of ART1 expression in CD4+ CD39+ T cells than the other subtypes studied. Neither shedding of CD62L nor cell death of CD39+ Treg or CD39- Treg cells was observed by 1mM ATP or 60 mu M NAD. In contrast, P2Xs receptor-dependent proliferation with 300 p,M ATP, was inhibited by NAD in the different cell types analysed. The NAD proliferation-inhibition was increased with P2X5 and A2a agonist and was reversed with P2X5 and A2a antagonist, therefore NAD inhibits P2Xs-dependent proliferation and A2a activation. In conclusion, our results suggest that the altered function and expression of P2X7 and ART1 in the human CD39+ Treg or CD39 Treg cells could participate in the resistance against cell death induced by ATP or NAD. (C) 2015 Elsevier GmbH. All rights reserved." | |
| Elsevier | |
| 2016 | |
| Artículo | |
| Juan D. Cortés-Garcia, Cintya López-López, Nancy Cortez-Espinosa, Mariana H. García-Hernández, Juan M. Guzmán-Flores, Esther Layseca-Espinosa, Liliana Portales-Cervantes, Diana P. Portales-Pérez, Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets, Immunobiology, Volume 221, Issue 1, 2016, Pages 84-93. | |
| BIOLOGÍA MOLECULAR | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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