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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1953| The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse | |
| Elizabeth Monreal Escalante GABRIELA NAVARRO TOVAR AMALIA FERNANDA LEON GALLO Margarita Juárez Montiel ALICIA BECERRA FLORA JUAN FRANCISCO JIMENEZ BREMONT SERGIO ROSALES MENDOZA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.jbiotec.2016.04.047 | |
| Cholera Low-cost vaccine Oral immunization Cold chain Neutralizing antibodies Cholera toxin | |
| "The use of corn smut for the production of recombinant vaccines has been recently implemented by our group. In this study, the stability and immunogenic properties of the corn smut-based cholera vaccine, based on the cholera toxin B subunit (CTB), were determined in mouse. The immunogenic potential of distinct corn smut CTB doses ranging from 1 to 30 ?g were assessed, with maximum humoral responses at both the systemic (IgG) and intestinal (IgA) levels at a dose of 15 ?g. The humoral response last for up to 70 days after the third boost. Mice were fully protected against a challenge with cholera toxin after receiving three 15 ?g-doses. Remarkably, the corn smut-made vaccine retained its immunogenic activity after storage at room temperature for a period of 1 year and no reduction on CTB was observed following exposure at 50 °C for 2 h. These data support the use of the corn smut-made CTB vaccine as a highly stable and effective immunogen and justify its evaluation in target animal models, such as piglet and sheep, as well as clinical evaluations in humans." | |
| Elsevier | |
| 2016 | |
| Artículo | |
| Elizabeth Monreal-Escalante, Gabriela Navarro-Tovar, Amalia León-Gallo, Margarita Juárez-Montiel, Alicia Becerra-Flora, Juan Francisco Jiménez-Bremont, Sergio Rosales-Mendoza, The corn smut-made cholera oral vaccine is thermostable and induces long-lasting immunity in mouse, Journal of Biotechnology, Volume 234, 2016, Pages 1-6. | |
| MICROBIOLOGÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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