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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2090| Diversification of transcriptional regulation determines subfunctionalization of paralogous branched chain aminotransferases in the yeast Saccharomyces cerevisiae | |
| James González Geovani Lopez Ortiz FULVIA STEFANY ARGUETA ZEPEDA XIMENA MARTINEZ DE LA ESCALERA FANJUL mohamed hafidi JOSE CARLOS CAMPERO BASALDUA Joseph| Strauss Lina Raquel Riego Ruiz MARIA ALICIA GONZALEZ MANJARREZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1534/genetics.117.300290 | |
| Functional diversification Expression regulation Aminotransferases Amino acid metabolism Paralogous genes | |
| "Saccharomyces cerevisiae harbors BAT1 and BAT2 paralogous genes that encode branched chain aminotransferases and have opposed expression profiles and physiological roles . Accordingly, in primary nitrogen sources such as glutamine, BAT1 expression is induced, supporting Bat1-dependent valine–isoleucine–leucine (VIL) biosynthesis, while BAT2 expression is repressed. Conversely, in the presence of VIL as the sole nitrogen source, BAT1 expression is hindered while that of BAT2 is activated, resulting in Bat2-dependent VIL catabolism. The presented results confirm that BAT1 expression is determined by transcriptional activation through the action of the Leu3–?-isopropylmalate (?-IPM) active isoform, and uncovers the existence of a novel ?-IPM biosynthetic pathway operating in a put3? mutant grown on VIL, through Bat2-Leu2-Leu1 consecutive action. The classic ?-IPM biosynthetic route operates in glutamine through the action of the leucine-sensitive ?-IPM synthases. The presented results also show that BAT2 repression in glutamine can be alleviated in a ure2? mutant or through Gcn4-dependent transcriptional activation. Thus, when S. cerevisiae is grown on glutamine, VIL biosynthesis is predominant and is preferentially achieved through BAT1; while on VIL as the sole nitrogen source, catabolism prevails and is mainly afforded by BAT2." | |
| Genetics Society of America | |
| 2017 | |
| Artículo | |
| González, J., López, G., Argueta, S., Escalera-Fanjul, X., El Hafidi, M., Campero-Basaldua, C., … González, A. (2017). Diversification of Transcriptional Regulation Determines Subfunctionalization of Paralogous Branched Chain Aminotransferases in the Yeast Saccharomyces cerevisiae. Genetics, 207(3), 975–991. doi:10.1534/genetics.117.300290 | |
| GENÉTICA | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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