Por favor, use este identificador para citar o enlazar este ítem:
http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2098| Constitutive expression of the active fragment of human vasostatin Vs30 in Pichia pastoris SMD1168H | |
| SERGIO CALDERON SALAIS PRISILIANA VELAZQUEZ BERNARDINO VICTOR EMMANUEL BALDERAS HERNANDEZ Ana Paulina Barba de la Rosa Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.pep.2017.12.002 | |
| Angiogenesis Constitutive expression Multi-copy expression strain Spheroids Vasostatin | |
| "Vasostatin 30 (Vs30) is an active fragment derived from the N-terminal region (135–164 aa) of human calreticulin and has the ability to inhibit angiogenesis. In this work, the expression of Vs30 was performed using a protease-deficient strain of the methylotrophic yeast Pichia pastoris. The vs30 gene was optimized for P. pastoris preferential codon usage and inserted into constitutive expression vector pGAPZ?A. In addition, a plasmid with four copies of the expression cassette was obtained and transformed into P. pastoris. The flask fermentation conditions were: culture volume of 25 mL in 250 mL baffled flasks at 28 °C, pH 6 and harvest time of 48 h. Up to 21.07 mg/L Vs30 were attained and purified by ultrafiltration with a 30-kDa cut-off membrane and the recovery was 49.7%. Bioactivity of Vs30 was confirmed by the inhibition of cell proliferation, as well as the inhibition of the capillary-like structures formation of EA.hy926 cells in vitro. This work constitutes the first report on the expression of Vs30 in Pichia pastoris using a constitutive promoter and multi-copy approach such as strategies to improve the recombinant Vs30 expression." | |
| Elsevier | |
| 2018 | |
| Artículo | |
| Sergio Calderon-Salais, Prisiliana Velazquez-Bernardino, Victor E. Balderas-Hernandez, Ana P. Barba de la Rosa, Antonio De Leon-Rodriguez, Constitutive expression of the active fragment of human vasostatin Vs30 in Pichia pastoris SMD1168H, Protein Expression and Purification, Volume 144, 2018, Pages 40-45., https://doi.org/10.1016/j.pep.2017.12.002 | |
| BIOQUÍMICA | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| ProteinExpresPurif144(2018)40.pdf | 696.65 kB | Adobe PDF | Visualizar/Abrir |