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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2117| Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions | |
| NANCY EDITH LOZOYA PEREZ J Sergio Casas Flores JOSE ASCENCION MARTINEZ ALVAREZ Luz Adriana López Ramírez Leila Maria Lópes Bezerra Bernardo Franco HECTOR MANUEL MORA MONTES | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.funbio.2018.07.004 | |
| Agrobacterium tumefaciens Bioimaging Cell wall Immune sensing Transformation Virulence | |
| "Sporotrichosis is an infection caused by members of the Sporothrix genus, and among them, Sporothrix schenckii is one of the etiological agents. Both, the disease and the causative agent have gained interest in the recent years, because of the report of epidemic outbreaks, and the description of the disease transmission from animals to human beings. Despite the relevance of S. schenckii in the clinical field, there are basic aspects of its biology poorly explored. So far, Agrobacterium tumefaciens-mediated transformation has been reported as an alternative for genetic manipulation of this fungal pathogen. Here, we report the optimization of the transformation method and used this to generate insertional mutants that express the green fluorescent protein in S. schenckii. We obtained five mutant strains that showed mitotic stability and expression of the reporter gene. The strains displayed normal cell wall composition, and a similar ability to interact ex vivo with human monocytes and monocyte-derived macrophages. Moreover, the virulence in larvae of Galleria mellonella was similar to that obtained with the wild-type control strains. These data indicate that these fluorescent mutants with normal ability to interact with the host could be used in bioimaging to track the host-Sporothrix interaction in vivo." | |
| Elsevier | |
| 2018 | |
| Artículo | |
| Nancy E. Lozoya-Pérez, Sergio Casas-Flores, José A. Martínez-Álvarez, Luz A. López-Ramírez, Leila M. Lopes-Bezerra, Bernardo Franco, Héctor M. Mora-Montes, Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions, Fungal Biology, Volume 122, Issue 10, 2018, Pages 1023-1030. | |
| MICOLOGÍA (SETAS) | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| FungalBiology122(2018)1023.pdf | 1.43 MB | Adobe PDF | Visualizar/Abrir |