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Defining novel plant polyamine oxidase subfamilies through molecular modeling and sequence analysis
César Daniel Bordenave
CAROLINA GRANADOS MENDOZA
JUAN FRANCISCO JIMENEZ BREMONT
Andrés Gárriz
Andrés Rodríguez
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.1186/s12862-019-1361-z
Evolution
Phylogeny
Polyamine oxidase
Polyamine catabolism
Protein structure
Homology modeling
"Background The polyamine oxidases (PAOs) catabolize the oxidative deamination of the polyamines (PAs) spermine (Spm) and spermidine (Spd). Most of the phylogenetic studies performed to analyze the plant PAO family took into account only a limited number and/or taxonomic representation of plant PAOs sequences. Results Here, we constructed a plant PAO protein sequence database and identified four subfamilies. Subfamily PAO back conversion 1 (PAObc1) was present on every lineage included in these analyses, suggesting that BC-type PAOs might play an important role in plants, despite its precise function is unknown. Subfamily PAObc2 was exclusively present in vascular plants, suggesting that t-Spm oxidase activity might play an important role in the development of the vascular system. The only terminal catabolism (TC) PAO subfamily (subfamily PAOtc) was lost in Superasterids but it was present in all other land plants. This indicated that the TC-type reactions are fundamental for land plants and that their function could being taken over by other enzymes in Superasterids. Subfamily PAObc3 was the result of a gene duplication event preceding Angiosperm diversification, followed by a gene extinction in Monocots. Differential conserved protein motifs were found for each subfamily of plant PAOs. The automatic assignment using these motifs was found to be comparable to the assignment by rough clustering performed on this work. Conclusions The results presented in this work revealed that plant PAO family is bigger than previously conceived. Also, they delineate important background information for future specific structure-function and evolutionary investigations and lay a foundation for the deeper characterization of each plant PAO subfamily."
BMC
2019
Artículo
Bordenave, C.D., Granados Mendoza, C., Jiménez Bremont, J.F. et al. Defining novel plant polyamine oxidase subfamilies through molecular modeling and sequence analysis. BMC Evol Biol 19, 28 (2019). https://doi.org/10.1186/s12862-019-1361-z
BIOLOGÍA MOLECULAR
Versión publicada
publishedVersion - Versión publicada
Aparece en las colecciones: Publicaciones Científicas Biología Molecular

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