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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2137| Genome-wide identification of mango (Mangifera indica L.) polygalacturonases: expression analysis of family members and total enzyme activity during fruit ripening | |
| MITZUKO DAUTT CASTRO ANDRES GUADALUPE LOPEZ VIRGEN ADRIAN OCHOA LEYVA Carmen Arminda Contreras Vergara ANA PAULINA SORTILLON SORTILLON MIGUEL ANGEL MARTINEZ TELLEZ GUSTAVO ADOLFO GONZALEZ AGUILAR J Sergio Casas Flores JOSEFA ADRIANA SAÑUDO BARAJAS David N. Kuhn María Auxiliadora Islas Osuna | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.3389/fpls.2019.00969 | |
| Polygalacturonase Mangifera indica L. Gene expression Enzymatic activity Firmness Ripening | |
| "Mango (Mangifera indica L.) is an important commercial fruit that shows a noticeable loss of firmness during ripening. Polygalacturonase (PG, E.C. 3.2.1.15) is a crucial enzyme for cell wall loosening during fruit ripening since it solubilizes pectin and its activity correlates with fruit softening. Mango PGs were mapped to a genome draft using seventeen PGs found in mango transcriptomes and 48 bonafide PGs were identified. The phylogenetic analysis suggests that they are related to Citrus sinensis, which may indicate a recent evolutive divergence and related functions with orthologs in the tree. Gene expression analysis for nine PGs showed differential expression for them during post-harvest fruit ripening, MiPG21-1, MiPG14, MiPG69-1, MiPG17, MiPG49, MiPG23-3, MiPG22-7, and MiPG16 were highly up-regulated. PG enzymatic activity also increased during maturation and these results correlate with the loss of firmness observed in mango during post-harvest ripening, between the ethylene production burst and the climacteric peak. The analysis of PGs promoter regions identified regulatory sequences associated to ripening such as MADS-box, ethylene regulation like ethylene insensitive 3 (EIN3) factors, APETALA2-like and ethylene response element factors. During mango fruit ripening the action of at least these nine PGs contribute to softening, and their expression is regulated at the transcriptional level. The prediction of the tridimensional structure of some PGs showed a conserved parallel beta-helical fold related to polysaccharide hydrolysis and a modular architecture, where exons correspond to structural elements. Further biotechnological approaches could target specific softening-related PGs to extend mango post-harvest shelf life." | |
| Frontiers Media S.A. | |
| 2019 | |
| Artículo | |
| Dautt-Castro M, López-Virgen AG, Ochoa-Leyva A, Contreras-Vergara CA, Sortillón-Sortillón AP, Martínez-Téllez MA, González-Aguilar GA, Casas-Flores JS, Sañudo-Barajas A, Kuhn DN and Islas-Osuna MA (2019) Genome-Wide Identification of Mango (Mangifera indica L.) Polygalacturonases: Expression Analysis of Family Members and Total Enzyme Activity During Fruit Ripening. Front. Plant Sci. 10:969. doi: 10.3389/fpls.2019.00969 | |
| BIOLOGÍA MOLECULAR | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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