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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2143| The Ustilago maydis null mutant strains of the RNA-binding protein UmRrm75 accumulate hydrogen peroxide and melanin | |
| ALMA LAURA RODRIGUEZ PIÑA Margarita Juárez Montiel Itzell Eurídice Hernández Sánchez Aída Araceli Rodríguez Hernández FRANCISCO ELIHU BAUTISTA REDONDA ALICIA BECERRA FLORA EDGAR OLIVER LOPEZ VILLEGAS JUAN FRANCISCO JIMENEZ BREMONT | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1038/s41598-019-47133-4 | |
| "Ustilago maydis is a dimorphic fungus that has emerged as a model organism for the study of fungal phytopathogenicity and RNA biology. In a previous study, we isolated the U. maydis UmRrm75 gene. The deletion of the UmRrm75 gene affected morphogenesis and pathogenicity. UmRrm75 gene encodes a protein containing three RNA recognition motifs. Here we determined that UmRrm75 has chaperone activity in Escherichia coli using the transcription anti-termination assay. Subsequently, we analyzed the growth of Delta UmRrm75 mutants at 15 degrees C and 37 degrees C, observing that mutant strains had reduced growth in comparison to parental strains. UmRrm75 gene expression was induced under these non-optimal temperatures. Delta UmRrm75 mutant colonies displayed a dark-brown color at 28 degrees C, which was confirmed to be melanin based on spectroscopic analysis and spectrometric data. Furthermore, Delta UmRrm75 mutant strains showed the presence of peroxisomes, and increased H2O2 levels, even at 28 degrees C. The Delta UmRrm75 mutant strains displayed a higher expression of redox-sensor UmYap1 gene and increased catalase activity than the parental strains. Our data show that deletion of the UmRrm75 gene results in higher levels of H2O2, increased melanin content, and abiotic stress sensitivity." | |
| Nature Publishing Group | |
| 2019 | |
| Artículo | |
| Rodríguez-Piña, A.L., Juárez-Montiel, M., Hernández-Sánchez, I.E. et al. The Ustilago maydis null mutant strains of the RNA-binding protein UmRrm75 accumulate hydrogen peroxide and melanin. Sci Rep 9, 10813 (2019). https://doi.org/10.1038/s41598-019-47133-4 | |
| CIENCIAS TECNOLÓGICAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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