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Analysis of bacterial communities of infected primary teeth in a Mexican population
ENID KARINA SALAS LOPEZ
J Sergio Casas Flores
NGUYEN ESMERALDA LOPEZ LOZANO
ESTHER LAYSECA ESPINOSA
CHRISTIAN ALBERTO GARCIA SEPULVEDA
PERLA DEL CARMEN NIÑO MORENO
AMAURY DE JESUS POZOS GUILLEN
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.4317/medoral.23689
Pyrosequencing
Deciduous teeth
Oral bacterial microbiota
16S Rrna
Taxonomy
"Background: The objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing. Material and Methods: Microbiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed. Results: Ribosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well. Conclusions: The infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalent."
Medicina Oral S.L
2020
Artículo
Salas-López EK, Casas-Flores S, López-Lozano NE, Layseca-Espinosa E, García-Sepúlveda CA, Niño-Moreno PdC, et al. Analysis of bacteri al communities of infected primary teeth in a Mexican population. Med Oral Patol Oral Cir Bucal. 2020 Sep 1;25 (5):e668-74.
MEDICINA Y CIENCIAS DE LA SALUD
Versión publicada
publishedVersion - Versión publicada
Aparece en las colecciones: Publicaciones Científicas Biología Molecular

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