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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2468| Diisononyl Phthalate differentially affects sirtuin expression in the HepG2 cell line | |
| ANA KAREN GUTIERREZ GARCIA Mahua Choudhury Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1021/acs.chemrestox.9b00206 | |
| Peptides and proteins Genetics Free radicals Organic compounds Oxidative stress | |
| "Human exposure to phthalates has received special attention due to their possible adverse human health effects. Diisononyl phthalate (DINP) is a plasticizer still widely used in many products, despite being considered an endocrine disruptor. In this study, we evaluated DINP’s cytotoxicity, its effect on the levels of reactive oxygen species (ROS), and its effect on sirtuin expression in HepG2 cells. Results showed that 1 μg/mL DINP significantly downregulated Sirt1, Sirt2, Sirt3, and Sirt5 gene expression (p < 0.05), while other sirtuins remained unaffected. Furthermore, protein levels of Sirt1 and Sirt3 were significantly downregulated by 1 μg/mL DINP. On the other hand, 100 μg/mL DINP doubled the levels of lysine acetylation proteins (increased 2-fold) as well as reactive oxygen species (ROS) compared with the controls. In conclusion, our study suggests, for the first time, that DINP regulates the potential epigenetic disruptor sirtuin family and leads to induction of ROS via sirtuins." | |
| American Chemical Society | |
| 2019 | |
| Artículo | |
| Gutiérrez-García AK, Choudhury M, De Leon-Rodriguez A. Diisononyl Phthalate Differentially Affects Sirtuin Expression in the HepG2 Cell Line. Chem Res Toxicol. 2019 Sep 16;32(9):1863-1870. doi: 10.1021/acs.chemrestox.9b00206. | |
| FARMACOLOGÍA | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| ChemResToxicol32(2019)1863.pdf | 1.01 MB | Adobe PDF | Visualizar/Abrir |