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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2476| Scale-up of hydrogen and ethanol co-production by an engineered Escherichia coli | |
| ANGEL MARIO LOPEZ HIDALGO VICTOR EMMANUEL BALDERAS HERNANDEZ Antonio de León Rodríguez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.fuel.2021.121002 | |
| Hemicellulosic hydrolysates Dark fermentation Metabolic engineering Biofuels Phage P1 transduction | |
| "In this work, the scale-up from 0.01 to 10 L process for the co-production of hydrogen (H2) and ethanol (EtOH) by a genetically engineered Escherichia coli that utilizes hemicellulosic hydrolysates from wheat straw pretreated (WSP) as substrate is presented. Co-production of biofuels was performed through the redirection of carbon-flux to ethanol by deleting ldhA (D-lactate dehydrogenase) and frdD (fumarate reductase) genes in an H2-overproducer strain (E. coli WDH). Resulting strain, E. coli WDH-LF (Delta hycA Delta frdD Delta ldhA), increased up to 70% and 167% the H2 and EtOH production from glucose compared with the parenteral strain. Using WSP as substrate, the yields of H2 and EtOH remained constant at all the evaluated scales. In 10 L bioreactors, the production parameters such as maximum production, production rate and yield were 5,603.0 +/- 233.5 mL H2/L, 41.4 +/- 4.0 mL H2/L/h, 342.7 +/- 14.3 mL H2/g TRS, 7.90 +/- 0.28 g EtOH/L and 0.48 +/- 0.01 g EtOH/g TRS, respectively. The results demonstrate the potential of the co-production of H2 and EtOH at different production scales by the engineered E. coli strain using lignocellulosic biomass as feedstock, such as wheat straw hydrolysates." | |
| Elsevier | |
| 2021 | |
| Artículo | |
| Angel M. Lopez-Hidalgo, Victor E. Balderas Hernandez, Antonio De Leon-Rodriguez, Scale-up of hydrogen and ethanol co-production by an engineered Escherichia coli, Fuel, Volume 300, 2021, 121002, https://doi.org/10.1016/j.fuel.2021.121002. | |
| INGENIERÍA Y TECNOLOGÍA | |
| Versión revisada | |
| submittedVersion - Versión revisada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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|---|---|---|---|
| Fuel300(2021)121002.pdf | 1.07 MB | Adobe PDF | Visualizar/Abrir |