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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2504| Long-insert clone experimental evidence for assembly improvement and chimeric chromosomes detection in an allopentaploid beer yeast | |
| CINTIA NOEMI GOMEZ MUÑOZ LUIS FERNANDO GARCIA ORTEGA Javier Israel Montalvo Arredondo Esmeralda Pérez Ortega LUIS CASTULO DAMAS BUENROSTRO Lina Raquel Riego Ruiz | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1093/g3journal/jkab088 | |
| Lager Yeast Hybrid Genome BAC Assembly Translocations | |
| "Lager beer is made with the hybrid Saccharomyces pastorianus. Many publicly available S. pastorianus genome assemblies are highly fragmented due to the difficulties of assembling hybrid genomes, such as the presence of homeologous chromosomes from both parental types, and translocations between them. To improve the assembly of a previously sequenced lager yeast hybrid Saccharomyces sp. 790 and elucidate its genome structure, we proposed the use of alternative experimental evidence. We determined the phylogenetic position of Saccharomyces sp. 790 and established it as S. pastorianus 790. Then, we obtained from this yeast a bacterial artificial chromosome (BAC) genomic library with its BAC-end sequences (BESs). To analyze these data, we developed a pipeline (applicable to other assemblies) that classifies BES pairs alignments according to their orientation. For the case of S. pastorianus 790, paired-end BESs alignments validated parts of the assembly and unpaired-end ones suggested contig joins or misassemblies. Importantly, the BACs library was preserved and used for verification experiments. Unpaired-end alignments were used to upgrade the previous assembly and provided an improved detection of translocations. With this, we proposed a genome structure of S. pastorianus 790, which was similar to that of other lager yeasts; however, when we estimated chromosome copy number and experimentally measured its genome size, we discovered that one key difference is the outstanding S. pastorianus 790 ploidy level (allopentaploid). Altogether, our results show the value of combining bioinformatic analyses with experimental data such as long-insert clone information to improve a short-read assembly of a hybrid genome." | |
| Oxford University Press | |
| 2021 | |
| Artículo | |
| Cintia Gómez-Muñoz, Luis Fernando García-Ortega, Javier Montalvo-Arredondo, Esmeralda Pérez-Ortega, Luis Cástulo Damas-Buenrostro, Lina Riego-Ruiz, Long-insert clone experimental evidence for assembly improvement and chimeric chromosomes detection in an allopentaploid beer yeast, G3 Genes|Genomes|Genetics, Volume 11, Issue 7, July 2021, jkab088, https://doi.org/10.1093/g3journal/jkab088 | |
| GENÉTICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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