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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2546| Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA | |
| Itxaso Anso Luis GM Basso Lei Wang Alberto Marina Edgar Daniel Páez Pérez Christian Jäger Floriane Gavotto Montse Tersa Sebastián Perrone F-Xabier Contreras Jacques Prandi Martine GILLERON Carole Linster Francisco Corzana Todd Lowary Beatriz Trastoy Marcelo Guerin | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1126/sciadv.abj4565 | |
| Bacillus-calmette guerin Phosphatidylinositol mannosides Mannosyltransferase pima Enzymatic acylation Structural basis Lipid-bilayer Biosynthesis Mycobacteria Recognition Dynamics | |
| "Glycolipids are prominent components of bacterial membranes that play critical roles not only in maintaining the structural integrity of the cell but also in modulating host-pathogen interactions. PatA is an essential acyltransferase involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIMs), key structural elements and virulence factors of Mycobacterium tuberculosis. We demonstrate by electron spin resonance spectroscopy and surface plasmon resonance that PatA is an integral membrane acyltransferase tightly anchored to anionic lipid bilayers, using a two-helix structural motif and electrostatic interactions. PatA dictates the acyl chain composition of the glycolipid by using an acyl chain selectivity “ruler.” We established this by a combination of structural biology, enzymatic activity, and binding measurements on chemically synthesized nonhydrolyzable acyl–coenzyme A (CoA) derivatives. We propose an interfacial catalytic mechanism that allows PatA to acylate hydrophobic PIMs anchored in the inner membrane of mycobacteria, through the use of water-soluble acyl-CoA donors." | |
| AAAS | |
| 2021 | |
| Artículo | |
| Anso, I., Basso, L. G. M., Wang, L., Marina, A., Páez-Pérez, E. D., Jäger, C., … Guerin, M. E. (2021). Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA. Science Advances, 7(42). https://doi.org/10.1126/sciadv.abj4565 | |
| CIENCIAS TECNOLÓGICAS | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| SciAdv7(2021)4565.pdf | 9.03 MB | Adobe PDF | Visualizar/Abrir |