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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2583| Improved Stability of Human CGI-58 Induced by Phosphomimetic S237E Mutation | |
| MIRIAM LIVIER LLAMAS GARCIA Edgar Daniel Páez Pérez CLAUDIA GUADALUPE BENITEZ CARDOZA GABRIELA MARGARITA MONTERO MORAN SAMUEL LARA GONZALEZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1021/acsomega.1c06872 | |
| Adipose triglyceride lipase Protein Binding Domain Solubility Prediction Lipolysis | |
| "In lipolysis, the activating function of CGI-58 is regulated by its interaction with perilipin 1 (PLIN1) localized on the lipid droplet (LD), and its release is controlled by phosphorylation. Once lipolysis is stimulated by catecholamines, protein kinase A (PKA)-mediated phosphorylation enables the dissociation of the CGI-58/PLIN1 complex, thereby recruiting adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) to initiate fatty acid release. It has been shown that mouse CGI-58 mutant S239E, which mimics the phosphorylation of this residue, is able to dissociate from the CGI-58/PLIN1 complex and activate ATGL. Here, we analyze the stabilizing effect on human CGI-58 of a triple tryptophan to alanine mutant (3WA) on the LD-binding motif, as well as a quadruple mutant in which the phosphomimetic S237E substitution was introduced to the 3WA construct (3WA/S237E). We found that tryptophan residues promote wild-type (WT) protein aggregation in solution since their substitution for alanine residues favors the presence of the monomer. Our experimental data showed increased thermal stability and solubility of 3WA/S237E protein compared to the 3WA mutant. Moreover, the 3WA/S237E protein showed proper folding and a functional binding site for oleoyl-CoA. The analysis of a bioinformatic three-dimensional (3D) model suggests an intramolecular interaction between the phosphomimetic glutamic acid and a residue of the α/β hydrolase core. This could explain the increased solubility and stability observed in the 3WA/S237E mutant and evidences the possible role of serine 237 phosphorylation." | |
| American Chemical Society | |
| 2022 | |
| Artículo | |
| Llamas-García ML, Páez-Pérez ED, Benitez-Cardoza CG, Montero-Morán GM, Lara-González S. Improved Stability of Human CGI-58 Induced by Phosphomimetic S237E Mutation. ACS Omega. 2022 Apr 5;7(15):12643-12653. doi: 10.1021/acsomega.1c06872. PMID: 35474805; PMCID: PMC9026008. | |
| QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Biología Molecular |
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| ACSOmega7(2022)12643.pdf | 3.64 MB | Adobe PDF | Visualizar/Abrir |