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http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2338| Antioxidant activity of a new multiflorane-type triterpene from seeds of Cucurbita Argyrosperma and their protective role on hydrogen peroxide induced oxidative stress | |
| ROSA MARTHA PEREZ GUTIERREZ ALETHIA MUÑIZ RAMIREZ José María Mota Flores ABRAHAM HERIBERTO GARCIA CAMPOY | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.31989/ffhd.v10i2.680 | |
| Cucurbita Argyrosperma Antioxidants Multiflorane Free radical scavenging Oxidative stress | |
| "Background: Cucurbita Argyrosperma seeds have acquired a reputation as an herbal remedy to treat various diseases because this plant is a predominant source of natural compounds with potent anti-inflammatory, antioxidant properties, and seed supplementation improves oxidative stress. Previous studies indicated that an imbalance between H2O2 production and elimination capacity is responsible for β-cell vulnerability, making β-cell a target susceptible to pathological disasters. This investigation aimed to evaluate the protective effects of one new multiflorane-type triterpene 3β-trans-caffeoyloxymultiflor-8-ene- 7α,12β, 18 β-triol (1) from MeOH extract from C. Argyrosperma, on rat pancreatic β cells (INS-1 cells) exposed to hydrogen peroxide (H2O2) induced oxidative stress conditions. Methods: The chemical structure of the novel triterpene, which was identified as 3β-trans-caffeoyloxymultiflor-8-ene- 7α,12β, 18 β-triol (1), was established based on the interpretation of spectroscopic analyses. The antioxidant activities of 1 were leaded by detect radical scavenging potential of 2,2-dyphenyl-1-picrylhydrazyl (DPPH) and 3.1 2,2′-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) ABTS. The assays were conducted on INS-1 cells line exposed to increasing concentrations of 1 at 5,10 and 20 µg/mL and H2O2 at 250 µM. Then, the experiments, cell viability, cell integrity ((LDH; lactate dehydrogenase release), mitochondrial function (ATP analysis), ROS formation, lipid peroxidation (MDA) and caspase-3, 9 activities were measured in the cells. We also determined the effect of 1 on antioxidant enzyme levels and cytotoxicity in pancreatic β cells under oxidant conditions. Results: The results showed that triterpene displayed high free-radical-scavenging activity, which is similar to that of standard antioxidants used. At concentrations of 5, 10, and 20 𝜇g/mL protect INS-1 cells against H2O2 induced cytotoxicity decrease in cell death, with a marked increase in cell viability, sustained cellular functionality (ATP). Antioxidant enzymes such as glutathione peroxidase (GPx), glutathione reduced (GSH), catalase (CAT), superoxide dismutase (SOD), and the non-antioxidant enzyme (GSH) increased in INS-1 cells with 1 pretreatment. MDA in pancreatic cells was ameliorated by 1 pretreatment reducing intracellular reactive oxygen species level." | |
| Functional Food Center Inc. | |
| 2020 | |
| Artículo | |
| Gutierrez R.M.P., Ramirez A.M., Flores J.M.M., Campoy A.H.G. Antioxidant activity of a new multiflorane-type triterpene from Cucurbita argyrosperma seeds and their protective role in hydrogen peroxide induced oxidative stress. Functional Foods in Health and Disease 2020; 10(2): 95-111. DOI: https:/doi.org/10.31989/ffhd.v10i2.680 | |
| BIOLOGÍA VEGETAL (BOTÁNICA) | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Ciencias Ambientales |
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