Por favor, use este identificador para citar o enlazar este ítem: http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2355
Standardized protocol for determination of biohydrogen potential
JULIAN CARRILLO REYES
Germán Buitrón
IVAN MORENO ANDRADE
Aida Cecilia Tapia Rodríguez
Rodolfo Palomo Briones
Elías Razo Flores
OSCAR AGUILAR JUAREZ
Jorge Arreola Vargas
Nicolas BERNET
Adriana Braga
Lucia Braga Nan
Elena Castelló
Lucile Chatellard
Claudia Etchebehere
Laura Fuentes
Elizabeth León Becerril
Hugo Óscar Méndez Acosta
Gonzalo Ruiz-Filippi
Estela Tapia Venegas
Eric TRABLY
Jorge Wenzel
Marcelo Zaiat
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.1016/j.mex.2019.11.027
Biohydrogen potential protocol
Automatic protocol
Dark fermentation
Heat-treated inoculum
Manual protocol
"Biohydrogen production potential (BHP) depends on several factors like inoculum source, substrate, pH, among many others. Batch assays are the most common strategy to evaluate such parameters, where the comparison is a challenging task due to the different procedures used. The present method introduces the first internationally validated protocol, evaluated by 8 independent laboratories from 5 different countries, to assess the biohydrogen potential. As quality criteria, a coefficient of variation of the cumulative hydrogen production (Hmax) was defined to be <15 %. Two options to run BHP batch tests were proposed; a manual protocol with periodic measurements of biogas production, needing conventional laboratory materials and analytical equipment for biogas characterization; and an automatic protocol, which is run in a device developed for online measurements of low biogas production. The detailed procedures for both protocol options are presented, as well as data validating them. The validation showed acceptable repeatability and reproducibility, measured as intra- and inter-laboratory coefficient of variation, which can be reduced up to 9 %."
Elsevier
2020
Artículo
Julián Carrillo-Reyes, Germán Buitrón, Iván Moreno-Andrade, Aida Cecilia Tapia-Rodríguez, Rodolfo Palomo-Briones, Elías Razo-Flores, Oscar Aguilar-Juárez, Jorge Arreola-Vargas, Nicolas Bernet, Adriana Ferreira Maluf Braga, Lucia Braga, Elena Castelló, Lucile Chatellard, Claudia Etchebehere, Laura Fuentes, Elizabeth León-Becerril, Hugo Oscar Méndez-Acosta, Gonzalo Ruiz-Filippi, Estela Tapia-Venegas, Eric Trably, Jorge Wenzel, Marcelo Zaiat, Standardized protocol for determination of biohydrogen potential, Methods X, Volume 7, 2020, 100754, https://doi.org/10.1016/j.mex.2019.11.027.
CIENCIAS TECNOLÓGICAS
Versión publicada
publishedVersion - Versión publicada
Aparece en las colecciones: Publicaciones Científicas Ciencias Ambientales

Cargar archivos:


Fichero Tamaño Formato  
MethodsX7(2020)100754.pdf1.29 MBAdobe PDFVisualizar/Abrir