Por favor, use este identificador para citar o enlazar este ítem:
http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2521| Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells | |
| EMMANUEL PINA JIMENEZ Fernando Calzada FRANCISCO ELIHU BAUTISTA REDONDA ROSA MARIA ORDOÑEZ RAZO Claudia Velázquez REYNA ELIZABETH BARBOSA CABRERA NORMAND GARCIA HERNANDEZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.3390/ijms221910516 | |
| Incomptine A Sesquiterpene lactone Decachaeta incompta Cytotoxic activity iTRAQ Apoptosis ROS production | |
| "Sesquiterpene lactones are of pharmaceutical interest due their cytotoxic and antitumor properties, which are commonly found within plants of several genera from the Asteraceae family such as the Decachaeta genus. From Decachaeta incompta four heliangolide, namely incomptines A-D have been isolated. In this study, cytotoxic properties of incomptine A (IA) were evaluated on four lymphoma cancer cell lines: U-937, Farage, SU-DHL-2, and REC-1. The type of cell death induced by IA and its effects on U-937 cells were analyzed based on its capability to induce apoptosis and produce reactive oxygen species (ROS) through flow cytometry with 4′,6-diamidino-2-phenylindole staining, dual annexin V/DAPI staining, and dichlorofluorescein 2′,7′-diacetate, respectively. A differential protein expression analysis study was carried out by isobaric tags for relative and absolute quantitation (iTRAQ) through UPLC-MS/MS. Results reveal that IA exhibited cytotoxic activity against the cell line U-937 (CC50 of 0.12 ± 0.02 μM) and the incubation of these cells in presence of IA significantly increased apoptotic population and intracellular ROS levels. In the proteomic approach 1548 proteins were differentially expressed, out of which 587 exhibited a fold-change ≥ 1.5 and 961 a fold-change ≤ 0.67. Most of these differentially regulated proteins are involved in apoptosis, oxidative stress, glycolytic metabolism, or cytoskeleton structuration." | |
| MDPI | |
| 2021 | |
| Artículo | |
| Pina-Jiménez, E.; Calzada, F.; Bautista, E.; Ordoñez-Razo, R.M.; Velázquez, C.; Barbosa, E.; García-Hernández, N. Incomptine A Induces Apoptosis, ROS Production and a Differential Protein Expression on Non-Hodgkin’s Lymphoma Cells. Int. J. Mol. Sci. 2021, 22, 10516. https://doi.org/10.3390/ijms221910516 | |
| BIOLOGÍA MOLECULAR | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Publicaciones Científicas Ciencias Ambientales |
Cargar archivos:
| Fichero | Tamaño | Formato | |
|---|---|---|---|
| IntJMolSci22(2021)10516.pdf | 2.11 MB | Adobe PDF | Visualizar/Abrir |